live-cell biosensor glosensor Search Results


live-cell biosensor glosensor  (Promega)
90
Promega live-cell biosensor glosensor
Effects of β-AR and a-AR ligands on cAMP production in B16F10 cells: ( A ) Representative image of cAMP measurements in real time using a <t>GloSensor</t> cAMP biosensor (bas, baseline). ( B ) Integrated cAMP responses computed as % of integrated forskolin response from tracings obtained in B16F10 cells stably expressing GloSensor-22F probe. Measurements were obtained in the absence or the presence of the β-AR agonist isoproterenol (ISO 1 μM), the antagonists propranolol and ICI 118,551 (PRO 10 μM, ICI 118,551 10 μM), isoproterenol plus either propranolol or ICI 118,551 (ISO 1 μM + PRO 10 μM or ICI118,551 10 μM), and the α2 agonists clonidine (CLO 1 μM) and ST91 (ST-91 1 μM). Data of three independent experiments are reported. * p < 0.05. ( C ) Concentration–response curves for the ligand-induced enhancement of cAMP production. Epinephrine (EPI), isoproterenol (ISO), norepinephrine (NE). EC 50 for ISO, EPI and NE was 4.6 nM, 45 nM and 284 nM, respectively.
Live Cell Biosensor Glosensor, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/live-cell biosensor glosensor/product/Promega
Average 90 stars, based on 1 article reviews
live-cell biosensor glosensor - by Bioz Stars, 2026-04
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split luciferase based glosensor camp biosensor assay  (Promega)
90
Promega split luciferase based glosensor camp biosensor assay
Effects of β-AR and a-AR ligands on cAMP production in B16F10 cells: ( A ) Representative image of cAMP measurements in real time using a <t>GloSensor</t> cAMP biosensor (bas, baseline). ( B ) Integrated cAMP responses computed as % of integrated forskolin response from tracings obtained in B16F10 cells stably expressing GloSensor-22F probe. Measurements were obtained in the absence or the presence of the β-AR agonist isoproterenol (ISO 1 μM), the antagonists propranolol and ICI 118,551 (PRO 10 μM, ICI 118,551 10 μM), isoproterenol plus either propranolol or ICI 118,551 (ISO 1 μM + PRO 10 μM or ICI118,551 10 μM), and the α2 agonists clonidine (CLO 1 μM) and ST91 (ST-91 1 μM). Data of three independent experiments are reported. * p < 0.05. ( C ) Concentration–response curves for the ligand-induced enhancement of cAMP production. Epinephrine (EPI), isoproterenol (ISO), norepinephrine (NE). EC 50 for ISO, EPI and NE was 4.6 nM, 45 nM and 284 nM, respectively.
Split Luciferase Based Glosensor Camp Biosensor Assay, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/split luciferase based glosensor camp biosensor assay/product/Promega
Average 90 stars, based on 1 article reviews
split luciferase based glosensor camp biosensor assay - by Bioz Stars, 2026-04
90/100 stars
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camp biosensor glosensor-22f  (Promega)
90
Promega camp biosensor glosensor-22f
Effects of β-AR and a-AR ligands on cAMP production in B16F10 cells: ( A ) Representative image of cAMP measurements in real time using a <t>GloSensor</t> cAMP biosensor (bas, baseline). ( B ) Integrated cAMP responses computed as % of integrated forskolin response from tracings obtained in B16F10 cells stably expressing GloSensor-22F probe. Measurements were obtained in the absence or the presence of the β-AR agonist isoproterenol (ISO 1 μM), the antagonists propranolol and ICI 118,551 (PRO 10 μM, ICI 118,551 10 μM), isoproterenol plus either propranolol or ICI 118,551 (ISO 1 μM + PRO 10 μM or ICI118,551 10 μM), and the α2 agonists clonidine (CLO 1 μM) and ST91 (ST-91 1 μM). Data of three independent experiments are reported. * p < 0.05. ( C ) Concentration–response curves for the ligand-induced enhancement of cAMP production. Epinephrine (EPI), isoproterenol (ISO), norepinephrine (NE). EC 50 for ISO, EPI and NE was 4.6 nM, 45 nM and 284 nM, respectively.
Camp Biosensor Glosensor 22f, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/camp biosensor glosensor-22f/product/Promega
Average 90 stars, based on 1 article reviews
camp biosensor glosensor-22f - by Bioz Stars, 2026-04
90/100 stars
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caspase 3/7 biosensor gls.devd  (Promega)
90
Promega caspase 3/7 biosensor gls.devd
Effects of β-AR and a-AR ligands on cAMP production in B16F10 cells: ( A ) Representative image of cAMP measurements in real time using a <t>GloSensor</t> cAMP biosensor (bas, baseline). ( B ) Integrated cAMP responses computed as % of integrated forskolin response from tracings obtained in B16F10 cells stably expressing GloSensor-22F probe. Measurements were obtained in the absence or the presence of the β-AR agonist isoproterenol (ISO 1 μM), the antagonists propranolol and ICI 118,551 (PRO 10 μM, ICI 118,551 10 μM), isoproterenol plus either propranolol or ICI 118,551 (ISO 1 μM + PRO 10 μM or ICI118,551 10 μM), and the α2 agonists clonidine (CLO 1 μM) and ST91 (ST-91 1 μM). Data of three independent experiments are reported. * p < 0.05. ( C ) Concentration–response curves for the ligand-induced enhancement of cAMP production. Epinephrine (EPI), isoproterenol (ISO), norepinephrine (NE). EC 50 for ISO, EPI and NE was 4.6 nM, 45 nM and 284 nM, respectively.
Caspase 3/7 Biosensor Gls.Devd, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/caspase 3/7 biosensor gls.devd/product/Promega
Average 90 stars, based on 1 article reviews
caspase 3/7 biosensor gls.devd - by Bioz Stars, 2026-04
90/100 stars
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glosensor  (Promega)
90
Promega glosensor
Comparison between ELISA assay and reporter assay.
Glosensor, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/glosensor/product/Promega
Average 90 stars, based on 1 article reviews
glosensor - by Bioz Stars, 2026-04
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pglosensortm cgmp  (Promega)
90
Promega pglosensortm cgmp
Comparison between ELISA assay and reporter assay.
Pglosensortm Cgmp, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pglosensortm cgmp/product/Promega
Average 90 stars, based on 1 article reviews
pglosensortm cgmp - by Bioz Stars, 2026-04
90/100 stars
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glosensor caspase 3/7  (Promega)
90
Promega glosensor caspase 3/7
Comparison between ELISA assay and reporter assay.
Glosensor Caspase 3/7, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/glosensor caspase 3/7/product/Promega
Average 90 stars, based on 1 article reviews
glosensor caspase 3/7 - by Bioz Stars, 2026-04
90/100 stars
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glosensor camp assay  (Promega)
90
Promega glosensor camp assay
Comparison between ELISA assay and reporter assay.
Glosensor Camp Assay, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/glosensor camp assay/product/Promega
Average 90 stars, based on 1 article reviews
glosensor camp assay - by Bioz Stars, 2026-04
90/100 stars
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Image Search Results


Effects of β-AR and a-AR ligands on cAMP production in B16F10 cells: ( A ) Representative image of cAMP measurements in real time using a GloSensor cAMP biosensor (bas, baseline). ( B ) Integrated cAMP responses computed as % of integrated forskolin response from tracings obtained in B16F10 cells stably expressing GloSensor-22F probe. Measurements were obtained in the absence or the presence of the β-AR agonist isoproterenol (ISO 1 μM), the antagonists propranolol and ICI 118,551 (PRO 10 μM, ICI 118,551 10 μM), isoproterenol plus either propranolol or ICI 118,551 (ISO 1 μM + PRO 10 μM or ICI118,551 10 μM), and the α2 agonists clonidine (CLO 1 μM) and ST91 (ST-91 1 μM). Data of three independent experiments are reported. * p < 0.05. ( C ) Concentration–response curves for the ligand-induced enhancement of cAMP production. Epinephrine (EPI), isoproterenol (ISO), norepinephrine (NE). EC 50 for ISO, EPI and NE was 4.6 nM, 45 nM and 284 nM, respectively.

Journal: International Journal of Molecular Sciences

Article Title: Crosstalk between β2- and α2-Adrenergic Receptors in the Regulation of B16F10 Melanoma Cell Proliferation

doi: 10.3390/ijms23094634

Figure Lengend Snippet: Effects of β-AR and a-AR ligands on cAMP production in B16F10 cells: ( A ) Representative image of cAMP measurements in real time using a GloSensor cAMP biosensor (bas, baseline). ( B ) Integrated cAMP responses computed as % of integrated forskolin response from tracings obtained in B16F10 cells stably expressing GloSensor-22F probe. Measurements were obtained in the absence or the presence of the β-AR agonist isoproterenol (ISO 1 μM), the antagonists propranolol and ICI 118,551 (PRO 10 μM, ICI 118,551 10 μM), isoproterenol plus either propranolol or ICI 118,551 (ISO 1 μM + PRO 10 μM or ICI118,551 10 μM), and the α2 agonists clonidine (CLO 1 μM) and ST91 (ST-91 1 μM). Data of three independent experiments are reported. * p < 0.05. ( C ) Concentration–response curves for the ligand-induced enhancement of cAMP production. Epinephrine (EPI), isoproterenol (ISO), norepinephrine (NE). EC 50 for ISO, EPI and NE was 4.6 nM, 45 nM and 284 nM, respectively.

Article Snippet: Here, we used the live-cell biosensor GloSensor (Promega, Madison, WI, USA) to assess whether tumor β-ARs are coupled to the Gs-cAMP signaling pathway, that is, whether their stimulation causes an increase in cAMP levels.

Techniques: Stable Transfection, Expressing, Concentration Assay

Comparison between ELISA assay and reporter assay.

Journal: Journal of Pharmaceutical Analysis

Article Title: A rapid reporter assay for recombinant human brain natriuretic peptide (rhBNP) by GloSensor technology

doi: 10.1016/j.jpha.2018.04.003

Figure Lengend Snippet: Comparison between ELISA assay and reporter assay.

Article Snippet: In this study, a new reporter assay was explored based on GloSensor technology, a platform technology of biosensors for the intracellular detection of signal transduction in living cells developed by Promega Corporation , , .

Techniques: Comparison, Enzyme-linked Immunosorbent Assay, Reporter Assay, Competitive ELISA, Intra Assay, Inter Assay